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International Journal of Spectroscopy
Article . 2011 . Peer-reviewed
License: CC BY
Data sources: Crossref
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International Journal of Spectroscopy
Article
License: CC BY
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ZENODO
Article . 2011
License: CC BY
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Rapid Determination of Total Thujone in Absinthe Using 1H NMR Spectroscopy

Authors: Monakhova, Yulia B.; Kuballa, Thomas; Lachenmeier, Dirk W.;

Rapid Determination of Total Thujone in Absinthe Using 1H NMR Spectroscopy

Abstract

1H NMR spectroscopy is utilized to quantify total thujone (sum of α‐ and β‐isomers) in absinthe. For sample preparation, a simple dilution with buffer is required. Thujone produces a distinct peak of the CH2 group in the cyclopentanone moiety in the 2.13–2.11 ppm range. No overlap with other typical constituents such as anethole or fenchone occurs. The detection limit of 0.3 mg/L is adequate to control the EU maximum limit. The relative standard deviation was 6%, and linearity was observed from 1 to 100 mg/L. Applicability was proven by analysis of 69 authentic absinthes. The correlation between NMR and our previous method consisting of liquid‐liquid extraction followed by GC/MS was significant (P < 0.0001, R = 0.93). The simple and cheap NMR method can be used for rapid screening of absinthes for total thujone content while chromatographic techniques are recommended for more specific (α‐ and β‐thujone isomers) analysis if required.

Keywords

thujone, absinthe, NMR

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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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