Identification of the Iron-Responsive Element for the Translational Regulation of Human Ferritin mRNA

descriptionPublicationkeyboard_double_arrow_right Article 11 Dec 1987 English Publisher:American Association for the Advancement of Science (AAAS)Journal:Science, volume 238, pages 1,570-1,573 (issn: 0036-8075, eissn: 1095-9203,

Copyright policy )Funded by:NIH | TERMINAL DIFFERENTIATION ..., NIH | CORE COMPONENT, NIH | REGENERATION OF EPIDERMIS... +1 projects

Authors: Hentze, M.; Caughman, S.; Rouault, T.; Barriocanal, J.; Dancis, A.; Harford, J.; Klausner, R.;

doi: 10.1126/science.3685996

pmid: 3685996

Identification of the Iron-Responsive Element for the Translational Regulation of Human Ferritin mRNA

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Abstract

Regulated translation of messenger RNA offers an important mechanism for the control of gene expression. The biosynthesis of the intracellular iron storage protein ferritin is translationally regulated by iron. A cis -acting element that is both necessary and sufficient for this translational regulation is present within the 5′ nontranslated leader region of the human ferritin H-chain messenger RNA. In this report the iron-responsive element (IRE) was identified by deletional analysis. Moreover, a synthetic oligodeoxynucleotide was shown to be able to transfer iron regulation to a construct that would otherwise not be able to respond to iron. The IRE has been highly conserved and predates the evolutionary segregation between amphibians, birds, and man. The IRE may prove to be useful for the design of translationally regulated expression systems.

Related Organizations

National Institute of Health
Pakistan

Keywords

Base Sequence, Iron, Molecular Sequence Data, Gene Expression Regulation, Genes, Protein Biosynthesis, Ferritins, Humans, RNA, Messenger, Chromosome Deletion, Promoter Regions, Genetic, Plasmids

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