We have unveiled the first genomic profile of the ancient Türkic royal family and Chinese historical celebrities. Our genomic analyses of Empress Ashina revealed Göktürk’s Northeast Asian origin (97.7% Northeast Asian ancestry and 2.3% West Eurasian ancestry), refuting the western Eurasian origin and multiple origin hypotheses. We found Ashina shared most genetic affinity with post-Iron Age Tungusic and Mongolic Steppe pastoralists, such as Rouran, Xianbei, Khitan, and Heshui_Mohe, and showed genetic heterogeneity with other ancient Türkic people, suggesting the multiple sources of the Türkic Khanate populations. Furthermore, the limited contribution from ancient Göktürk found in modern Turkic speaking populations once again validates a cultural diffusion model over a demic diffusion model for the spread of Turkic languages.