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https://doi.org/10.1101/749069...
Article . 2019 . Peer-reviewed
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A template-matching algorithm for laminar identification of cortical recording sites from evoked response potentials

Authors: Matteucci, Giulio; Riggi, Margherita; Zoccolan, Davide;

A template-matching algorithm for laminar identification of cortical recording sites from evoked response potentials

Abstract

Abstract In recent years, the advent of the so-called silicon probes has made it possible to homogeneously sample spikes and local field potentials (LFPs) from a regular grid of cortical recording sites. In principle, this allows inferring the laminar location of the sites based on the spatiotemporal pattern of LFPs recorded along the probe, as in the well-known current source-density (CSD) analysis. This approach, however, has several limitations, since it relies on visual identification of landmark features (i.e., current sinks and sources) by human operators – features that can be absent from the CSD pattern if the probe does not span the whole cortical thickness, thus making manual labelling harder. Furthermore, as any manual annotation procedure, the typical CSD-based workflow for laminar identification of recording sites is affected by subjective judgment undermining the consistency and reproducibility of results. To overcome these limitations, we developed an alternative approach, based on finding the optimal match between the LFPs recorded along a probe in a given experiment and a template LFP profile that was computed using 18 recording sessions, in which the depth of the recording sites had been recovered through histology. We show that this method can achieve an accuracy of 79 µm in recovering the cortical depth of recording sites and a 76% accuracy in inferring their laminar location. As such, our approach provides an alternative to CSD that, being fully automated, is less prone to the idiosyncrasies of subjective judgment and works reliably also for recordings spanning a limited cortical stretch. New and noteworthy Knowing the depth and laminar location of the microelectrodes used to record neuronal activity from the cerebral cortex is crucial to properly interpret the recorded patterns of neuronal responses. Here we present an innovative approach that allows inferring such properties with high accuracy and in an automated way (i.e., without the need of visual inspection and manual annotation) from the evoked response potentials (ERPs) elicited by sensory (e.g., visual) stimuli.

Country
Italy
Keywords

Cortical layers, Silicon probes, Visually evoked potentials, Visual cortex

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
3
Average
Average
Average
Green