The Effect of Gentian Violet on the Bacillus Tetani, Tetanus Toxin and Certain Laboratory Animals

descriptionPublicationkeyboard_double_arrow_right Article 01 Nov 1914 English Publisher:Oxford University Press (OUP)Journal:Journal of Infectious Diseases, volume 15, pages 566-579 (issn: 0022-1899, eissn: 1537-6613,

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Authors: I. C. Hall; L. B. Taber;

doi: 10.1093/infdis/15.3.566

The Effect of Gentian Violet on the Bacillus Tetani, Tetanus Toxin and Certain Laboratory Animals

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Abstract

The use of phenol, or tricresol, or of toluene fails when applied to tetanus cultures, because of the resistance of the spores and the lability of the toxin. Sedimentation and centrifugalization are uncertain and unsafe even when a preservative is added. Berkefeld filtration with or without antiseptic is effective and safe only if extreme care is taken to prevent the spilling of the culture. One of us2 has devised an apparatus to facilitate safe separation of the culture from the oil used to exclude air from the medium.

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