A novel method for sample delivery and testing of whole blood: gel strip PCR for point of care (POC) molecular diagnostics

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2013 English Publisher:Royal Society of Chemistry (RSC)Journal:Lab on a Chip, volume 13, page 4,011 (issn: 1473-0197, eissn: 1473-0189,

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Authors: Manage, Dammika P.; Lauzon, Jana; Atrazhev, Alexey; Pang, Xiaoli; Pilarski, Linda M.;

doi: 10.1039/c3lc50755f

pmid: 23966268

A novel method for sample delivery and testing of whole blood: gel strip PCR for point of care (POC) molecular diagnostics

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Abstract

Testing of whole blood in miniaturized PCR is compromised by the opaque nature of whole blood that leads to physical masking of a fluorescent signal. We demonstrate a method to perform real-time PCR with whole blood that avoids interference from the opacity of whole blood.

Related Organizations

Cross Cancer Institute
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Cross Cancer Institute
Canada
University of Alberta
Canada
Provincial Laboratory of Public Health
Canada

Keywords

Point-of-Care Systems, Real-Time Polymerase Chain Reaction, Polymerase Chain Reaction, Blood, Molecular Diagnostic Techniques, Humans, Artifacts, Gels, Blood Chemical Analysis, Reagent Strips

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