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XTEND: Extending the depth of field in cryo soft X-ray tomography

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 04 Apr 2017 Spain English Publisher:Springer Science and Business Media LLCJournal:Scientific Reports, volume 7 (eissn: 2045-2322, Copyright policy )Funded by:NSF | Computational Methods for..., EC | iNEXT, EC | BIOSTRUCT-X
Authors: Otón, Joaquín; Pereiro, Eva; Conesa, José J.; Chichón, Francisco J.; Luque, Daniel; Rodríguez, Javier M.; Pérez-Berná, Ana J.; +7 Authors

doi: 10.1038/srep45808

pmid: 28374769

pmc: PMC5379191

handle: 20.500.12614/1347 , 20.500.12105/7365

XTEND: Extending the depth of field in cryo soft X-ray tomography

Abstract

AbstractWe have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard reconstruction are now recovered. This approach, based on focal series projections, is easily applicable with closed expressions to select specific data acquisition parameters.

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Keywords

Cell biology, Microscopy, Imaging, Three-Dimensional, Tomography, X-Ray, X-rays, Image Processing, Computer-Assisted, Structural biology, Article, Algorithms

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selected citations
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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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