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Biochemical and Biophysical Research Communications
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Biochemical and Biophysical Research Communications
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Biochemical and Biophysical Research Communications
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https://dx.doi.org/10.1016/j.b...
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Putative sperm fusion protein IZUMO and the role of N-glycosylation

descriptionPublicationkeyboard_double_arrow_right Article 01 Dec 2008 English Publisher:Elsevier BVJournal:Biochemical and Biophysical Research Communications, volume 377, pages 910-914 (issn: 0006-291X, Copyright policy )
Authors: Naokazu, Inoue; Masahito, Ikawa; Masaru, Okabe;

doi: 10.1016/j.bbrc.2008.10.073

pmid: 18952059

Putative sperm fusion protein IZUMO and the role of N-glycosylation

Abstract

IZUMO is the mouse sperm protein proven to be essential for fusion with eggs. It contains one immunoglobulin-like domain with a conserved glycosylation site within. In the present paper, we produced transgenic mouse lines expressing unglycosylated IZUMO (N204Q-IZUMO) in Izumo1 -/- background. The expression of N204Q-IZUMO rescued the infertile phenotype of IZUMO disrupted mice, indicating glycosylation is not essential for fusion-facilitating activity of IZUMO. The N204Q-IZUMO was produced in testis in comparable amounts to wild-type IZUMO, but the amount of N204Q-IZUMO on sperm was significantly decreased by the time sperm reached the cauda epididymis. These data suggest that glycosylation is not essential for the function of IZUMO, but has a role in protecting it from fragmentation in cauda epididymis.

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Keywords

Epididymis, Male, Glycosylation, Molecular Sequence Data, Immunoglobulins, Membrane Proteins, Mice, Transgenic, Spermatozoa, Rats, Cell Fusion, Mice, Dogs, Fertility, Animals, Humans, Female, Amino Acid Sequence, Ovum

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
views
OpenAIRE UsageCountsViews provided by UsageCounts
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63
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