Simulating natural conditions in the laboratory poses one of the most significant challenges to behavioral studies. Some authors have argued that laboratory "choice" experiments reflect mate choice in nature more accurately than "no-choice" experiments. A recent choice experiment study questioned the conclusions of several earlier studies by failing to detect a published difference in sexual isolation between populations of Drosophila pseudoobscura, and suggested their result was more robust because of the more realistic design. Here, we re-examine the methods and analyses of this recent study, and we find there was indeed a difference in sexual isolation between populations of D. pseudoobscura. We also conduct a more rigorously controlled choice experiment and, in agreement with previous studies, note that D. pseudoobscura females from populations sympatric to their sibling species, D. persimilis, exhibit greater sexual isolation than those from allopatric populations. Our results confirm the existence of a geographic pattern in sexual isolation in D. pseudoobscura, and we discuss differences in experimental designs in light of the biology of this species.