Phospholipid binding of antiphospholipid antibodies and placental anticoagulant protein

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1992 English Publisher:Springer Science and Business Media LLCJournal:Journal of Clinical Immunology, volume 12, pages 27-35 (issn: 0271-9142, eissn: 1573-2592,

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Authors: Sammaritano, Lisa R.; Gharavi, Azzudin E.; Soberano, Consolacion; Levy, Roger A.; Lockshin, Michael D.;

doi: 10.1007/bf00918270

pmid: 1372614

Phospholipid binding of antiphospholipid antibodies and placental anticoagulant protein

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Abstract

We evaluated the interaction of antiphospholipid antibodies (aPL) with placental anticoagulant protein I (PAP I), a calcium-dependent phospholipid binding protein which may act as a natural anticoagulant. Clotting assays showed additive prolongation of clotting times with aPL and PAP I. ELISA and vesicle phospholipid binding studies showed PAP I inhibition of aPL binding to phospholipid but no inhibition of PAP I-phospholipid binding by aPL. aPL and PAP I interact additively in anticoagulant activity in in vitro clotting systems and compete for phospholipid in ELISA system. These data support the hypotheses that aPL and PAP I may recognize similar phospholipid epitopes and that in vivo interaction may occur.

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Keywords

Dose-Response Relationship, Drug, Annexins, Placenta, Enzyme-Linked Immunosorbent Assay, In Vitro Techniques, Pregnancy Proteins, Antibodies, Calcium Chloride, Epitopes, Immunoglobulin G, Humans, Electrophoresis, Polyacrylamide Gel, Blood Coagulation Tests, Blood Coagulation, Phospholipids

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