Associations of UBE2I with RAD52, UBL1, p53, and RAD51 Proteins in a Yeast Two-Hybrid System

descriptionPublicationkeyboard_double_arrow_right Article 01 Oct 1996 English Publisher:Elsevier BVJournal:Genomics, volume 37, pages 183-186 (issn: 0888-7543,

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Authors: Z, Shen; P E, Pardington-Purtymun; J C, Comeaux; R K, Moyzis; D J, Chen;

doi: 10.1006/geno.1996.0540

pmid: 8921390

Associations of UBE2I with RAD52, UBL1, p53, and RAD51 Proteins in a Yeast Two-Hybrid System

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Abstract

The yeast RAD52-dependent pathway is involved in DNA recombination and double-strand break repair. Yeast ubiquitin-conjugating enzyme UBC9 participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the "bait" in a yeast two-hybrid system, we have identified a human homolog of yeast UBC9, designated UBE2I, that interacts with RAD52, RAD51, p53, and a ubiquitin-like protein UBL1. These interactions are UBE2I-specific, since another DNA repair-related ubiquitin-conjugating enzyme, RAD6 (UBC2), does not interact with these proteins. The interaction of UBE2I with RAD52 is mediated by RAD52's self-association region. These results suggest that the RAD52-dependent processes, cell cycle control, p53-mediated pathway(s), and ubiquitination interact through human UBE2I.

Related Organizations

Los Alamos National Laboratory
United States

Keywords

Male, Saccharomyces cerevisiae Proteins, DNA Repair, Molecular Sequence Data, SUMO-1 Protein, Chromosome Mapping, Saccharomyces cerevisiae, Rad52 DNA Repair and Recombination Protein, DNA-Binding Proteins, Fungal Proteins, Ligases, Testis, Humans, RNA, Messenger, Rad51 Recombinase, Schizosaccharomyces pombe Proteins, Tumor Suppressor Protein p53, Carrier Proteins, Chromosomes, Human, Pair 16, Protein Binding

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DNA repair protein RAD51
2017IsRelatedTo
DNA repair and recombination protein RAD52
2017IsRelatedTo
SUMO-conjugating enzyme UBC9
2017IsRelatedTo
Ubiquitin-conjugating enzyme E2 2
2017IsRelatedTo

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