AbstractWe have sequenced a gene that encodes a 377 amino acid putative protein with an ATPase motif typical of the protein family including SEC18p (NSF = N‐ethyl maleimide‐sensitive fusion protein; vesicle‐mediated endoplasmic reticulum to Golgi protein transfer), PAS1p (peroxisome assembly), CDC48p (VCP = valosin‐containing protein; cell cycle) and TBP1 (Tat‐binding protein). This gene, AFG1 for ATPase family, gene, also has substantial homology to these proteins outside the ATPase domain. AFG1 is located on chromosome V imediately centromere‐proximal to M AK10.