Abstract Global urbanisation reduces greenery and species richness (biodiversity) and limits opportunities for most children to gain experiences of nature. Disconnecting children from nature has implications for their play, health, well‐being, and comprehension of ecological systems, as well as their engagement with the environment as adults. In the competition for land for built infrastructure, the preservation of remaining greenery is essential to fulfill multiple functions. One way forward is to look for synergies between conservation of biodiversity and children's need for outdoor environments of high quality. In this paper, we synthesize the existing literature on how to understand the many interfaces between children and nature, suggesting perspectives and tools for the management and design of nature‐based play settings. We frame this transdisciplinary perspective using ‘play biotopes‘, as a conceptual framework in which both children's play and species are taken into account. We exemplify how the play biotope framework can be put into practice as part of (1) an overall approach to landscapes made more useful to both children and other species, (2) affordances for play in nature such as branches from dead wood, and (3) a design process of a playground by giving input to nature‐based solutions. A conclusion is that play biotopes as a conceptual framework within nature‐based solutions can help increase play and biodiversity by promoting structures for climbing, making huts and biotopes otherwise overlooked in urban planning. Read the free Plain Language Summary for this article on the Journal blog.