Listeria monocytogenes is a ubiquitous bacterium that causes the serious foodborne illness listeriosis. Although soil is a primary reservoir for Lm, the bacterium can be found in a variety of environments, including water, food, and the human gut. The ability of Lm to adapt to different environments and evade the host immune system is a key factor in its pathogenicity. The emergence of biocide-tolerant strains of Lm has raised concerns about the effectiveness of current control measures. This study aims to investigate the genomic epidemiology and strain taxonomy of Lm, with a focus on understanding the genetic mechanisms underlying its adaptability and virulence.