Carbapenem-resistant Enterobacteriaceae (CRE) pose a significant threat to public health worldwide. The emergence of carbapenemase-producing bacteria, such as Klebsiella pneumoniae, has led to the spread of CRE in healthcare settings. Understanding the molecular epidemiology, sequence types, and plasmid-mediated resistance mechanisms is crucial for developing effective control strategies. This research activity aims to investigate the clonal structure and resistance patterns of CRE in various healthcare settings, with a focus on the KPC-2-producing Klebsiella pneumoniae. The study will involve a multicenter approach, collecting and analyzing data from multiple hospitals and healthcare facilities. The findings of this research will provide valuable insights into the epidemiology and microbiology of CRE, ultimately informing the development of targeted interventions to combat the spread of these resistant bacteria.