A set of fluorene copolymers designed to introduce electron donor/acceptor structures in the polymer chain that could eventually produce more efficient charge separation with long lifetimes has been prepared. The photophysics of these copolymers is studied to understand the impact of the donor/acceptor structures on the charge separation process. The results show that the introduction of these structures leads to a significant improvement in the charge separation efficiency, paving the way for the development of more efficient organic optoelectronic devices.