A comparative kinematics study was conducted on six cadaver limbs, comparing tibiofemoral kinematics in five conditions: unloaded, under a constant 130 N ankle load with a variable quadriceps load, with the aim of understanding the influence of muscle load on the knee joint. The study aimed to provide insights into the biomechanical changes that occur in the knee joint under different loading conditions, which is essential for the development of effective treatment strategies for knee injuries and diseases.