To systematically identify and analyze the 15 HA and 9 NA subtypes of influenza A virus, we need reliable, simple methods that not only characterize partial sequences but analyze the entire influenza virus. This study aims to develop a universal primer set for the full-length amplification of the HA and NA genes of influenza A virus. The primer set will be validated using a panel of influenza A virus strains and will be used to analyze the genetic diversity of the HA and NA genes in different influenza A virus subtypes. The results of this study will provide valuable information on the genetic diversity of the HA and NA genes in different influenza A virus subtypes and will contribute to the development of effective diagnostic and therapeutic strategies for the prevention and treatment of influenza A virus infections.