Evidence for a pioneer round of mRNA translation: mRNAs subject to nonsense-mediated decay in mammalian cells are bound by CBP80 and CBP20.
Copyright policy )Evidence for a pioneer round of mRNA translation: mRNAs subject to nonsense-mediated decay in mammalian cells are bound by CBP80 and CBP20.
Nonsense-mediated decay (NMD) eliminates mRNAs that prematurely terminate translation. We used antibody to the nuclear cap binding protein CBP80 or its cytoplasmic counterpart eIF4E to immunopurify RNP containing nonsense-free or nonsense-containing transcripts. Data indicate that NMD takes place in association with CBP80. We defined other components of NMD-susceptible mRNP as CBP20, PABP2, eIF4G, and the NMD factors Upf2 and Upf3. Consistent with the dependence of NMD on translation, the NMD of CBP80-bound mRNA is blocked by cycloheximide or suppressor tRNA. These findings provide evidence that translation can take place in association with CBP80. They also indicate that CBP80-bound mRNA undergoes a "pioneer" round of translation, before CBP80-CBP20 are replaced by eIF4E, and Upf2 and Upf3 proteins dissociate from upstream of exon-exon junctions.
- University of Rochester United States
Cell Nucleus, Protein Synthesis Inhibitors, Glutathione Peroxidase, Macromolecular Substances, Immunoblotting, 3T3 Cells, Models, Biological, Poly(A)-Binding Proteins, Globins, Mice, Cross-Linking Reagents, Codon, Nonsense, Peptide Initiation Factors, Protein Biosynthesis, Alpha-Globulins, COS Cells, Animals, Humans, Cycloheximide, Eukaryotic Initiation Factor-4G
Cell Nucleus, Protein Synthesis Inhibitors, Glutathione Peroxidase, Macromolecular Substances, Immunoblotting, 3T3 Cells, Models, Biological, Poly(A)-Binding Proteins, Globins, Mice, Cross-Linking Reagents, Codon, Nonsense, Peptide Initiation Factors, Protein Biosynthesis, Alpha-Globulins, COS Cells, Animals, Humans, Cycloheximide, Eukaryotic Initiation Factor-4G
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