Endothelin (ET) is well known to be a potent vasoconstrictor peptide with autocrine and paracrine function. It has been documented that ET is also present in non-muscle tissues. The distribution of ET and ET-A receptor (ET-AR) in the monkey lacrimal gland was investigated by immunohistochemistry. Three adult male monkeys (Macaca fuscata) were perfused with a fixative. The lacrimal glands were then dissected and sectioned. Using rabbit anti-ET and anti-ET-AR antibodies, the immunohistochemical procedure was performed following an ABC technique. Some sections were treated with rhodamine-phalloidin, which selectively binds to actin filaments. ET immunoreactivity was present in stellate-shaped cells located around the alveoli. In sections double-stained with anti-ET antibody and rhodamine-phalloidin, ET immunoreactivity and abundant filamentous actin were identified in the same stellate cells. Immunostaining for ET-AR was also found in the stellate shape cells. The configuration of, and the abundance of actin filaments in the stellate-shaped ET- and ET-AR immunoreactive cells suggest that they are myoepithelial cells, which are contractile and may contribute to the process of lacrimal gland secretion or maintenance of the contour of the glandular endpieces. Our results indicate that endothelin is present in myoepithelial cells of the monkey lacrimal gland.