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Ethiopian Medical Journal

Article . 2005

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Evaluation of rapid HIV test kits on whole blood and development of rapid testing algorithm for voluntary testing and counseling centers in Ethiopia.

descriptionPublicationkeyboard_double_arrow_right Article 25 Oct 2005 English Journal:Ethiopian medical journal, volume 42 (issn: 0014-1755,

Authors: Belete, Tegbaru; Tsehaynesh, Messele; Dawit, Wolday; PhD Hailu, Meles; Desalegn, Tesema; Hiwot, Birhanu; Girma, Tesfaye; +5 Authors

pmid: 16122118

Evaluation of rapid HIV test kits on whole blood and development of rapid testing algorithm for voluntary testing and counseling centers in Ethiopia.

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Abstract

Five simple and rapid HIV antibody detection assays viz. Determine, Capillus, Oraquick, Unigold and Hemastrip were evaluated to examine their performance and to develop an alternative rapid test based testing algorithm for voluntary counseling and testing (VCT) in Ethiopia. All the kits were tested on whole blood, plasma and serum. The evaluation had three phases: Primary lab review, piloting at point of service and implementation. This report includes the results of the first two phases. A total of 2,693 specimens (both whole blood and plasma) were included in the evaluation. Results were compared to double Enzyme Linked Immuno-Sorbent Assay (ELISA) system. Discordant EIA results were resolved using Western Blot. The assays had very good sensitivities and specificities, 99-100%, at the two different phases of the evaluation. A 98-100% result agreement was obtained from those tested at VCT centers and National Referral Laboratory for AIDS (NRLA), in the quality control phase of the evaluation. A testing strategy yielding 100% [95% CI; 98.9-100.0] sensitivity was achieved by the sequential use of the three rapid test kits. Direct cost comparison showed serial testing algorithm reduces the cost of testing by over 30% compared to parallel testing in the current situation. Determine, Capillus/Oraquick (presence/absence of frefrigeration) and Unigold were recommended as screening, confirmation and tiebreaker tests, respectively.

Related Organizations

Ethiopian Public Health Institute
Ethiopia

Keywords

Time Factors, AIDS Serodiagnosis, Enzyme-Linked Immunosorbent Assay, HIV Infections, HIV Antibodies, Sensitivity and Specificity, Immunoenzyme Techniques, HIV Seronegativity, HIV Seropositivity, HIV-1, Humans, Ethiopia, Reagent Kits, Diagnostic, Algorithms

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