Influence of p53 status on the effects of boron neutron capture therapy in glioblastoma.
Copyright policy )Influence of p53 status on the effects of boron neutron capture therapy in glioblastoma.
The tumor suppressor gene p53 is mutated in glioblastoma. We studied the relationship between the p53 gene and the biological effects of boron neutron capture therapy (BNCT).The human glioblastoma cells; A172, expressing wild-type p53, and T98G, with mutant p53, were irradiated by the Kyoto University Research Reactor (KUR). The biological effects after neutron irradiation were evaluated by the cell killing effect, 53BP1 foci assay and apoptosis induction.The survival-fraction data revealed that A172 was more radiosensitive than T98G, but the difference was reduced when boronophenylalanine (BPA) was present. Both cell lines exhibited similar numbers of foci, suggesting that the initial levels of DNA damage did not depend on p53 function. Detection of apoptosis revealed a lower rate of apoptosis in the T98G.BNCT causes cell death in glioblastoma cells, regardless of p53 mutation status. In T98G cells, cell killing and apoptosis occurred effectively following BNCT.
- Kyoto University Japan
Cell Survival, Cell Line, Tumor, Intracellular Signaling Peptides and Proteins, Mutation, Missense, Humans, Apoptosis, Boron Neutron Capture Therapy, Tumor Suppressor Protein p53, Glioblastoma, Tumor Suppressor p53-Binding Protein 1
Cell Survival, Cell Line, Tumor, Intracellular Signaling Peptides and Proteins, Mutation, Missense, Humans, Apoptosis, Boron Neutron Capture Therapy, Tumor Suppressor Protein p53, Glioblastoma, Tumor Suppressor p53-Binding Protein 1
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