[FHL1 knockdown mediated by lentiviral shRNA promotes the growth of HeLa and HepG2 cells].
Copyright policy )[FHL1 knockdown mediated by lentiviral shRNA promotes the growth of HeLa and HepG2 cells].
To investigate the effect of four-and-a-half LIM domain 1 (FHL1) knockdown by lentiviral-mediated shRNA on the growth of HeLa and HepG2 cells.pLenti-H1 FHL1 shRNA was cloned, and then transfered into HEK293T cells. The inhibitory effect of pLenti-H1 FHL1 shRNA on FHL1 gene was detected by Western blotting and real-time quantitative PCR (qRT-PCR). Lentivirus particles were packaged, added to HeLa and HepG2 cells, followed by puromycin treatment for 2-3 weeks to screen stable clones. The knockdown effect on FHL1 expression in these cells was checked by Western blotting and qRT-PCR. Cell growth and colony formation analysis were performed to investigate the effect of FHL1 down-regulation on tumor cell growth. Soft agar analysis was used to analyze its effect on anchorage-independent tumor cell growth.Western blotting and qRT-PCR revealed that the pLenti-H1 FHL1 shRNA apparently inhibited the expression of FHL1 gene. Cell growth and colony formation assay showed that the lentiviral-mediated shRNA for FHL1 gene significantly accelerated the tumor cell growth in HepG2 and HeLa cells. Soft agar analysis demonstrated that FHL1 shRNA increased the anchorage-independent growth of tumor cells.pLenti-H1 FHL1 shRNA could significantly accelerate tumor cell growth via inhibiting the expression of FHL1.
Lentivirus, Intracellular Signaling Peptides and Proteins, Muscle Proteins, Hep G2 Cells, LIM Domain Proteins, Gene Expression Regulation, Neoplastic, Gene Knockdown Techniques, Neoplastic Stem Cells, Humans, RNA, Small Interfering, Cell Proliferation, HeLa Cells, Plasmids
Lentivirus, Intracellular Signaling Peptides and Proteins, Muscle Proteins, Hep G2 Cells, LIM Domain Proteins, Gene Expression Regulation, Neoplastic, Gene Knockdown Techniques, Neoplastic Stem Cells, Humans, RNA, Small Interfering, Cell Proliferation, HeLa Cells, Plasmids
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