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Thesis . 2011
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Regulation of the abscisic acid response by protein phosphatase 2C-interacting proteins ABP7 and ABP9 in Arabidopsis thaliana

Authors: Ma-Lauer, Yue;

Regulation of the abscisic acid response by protein phosphatase 2C-interacting proteins ABP7 and ABP9 in Arabidopsis thaliana

Abstract

The protein phosphatases 2C ABI1 and ABI2 are negative regulators in signal transduction of the phytohormone abscisic acid (ABA). The aim of this work is to characterize two homologous proteins ABP7 and ABP9, which were identified as interacting partners of ABI2 in the yeast two-hybrid system. In protoplasts, ABP7 and ABP9 interacted with both ABI1 and ABI2 in the nucleus and the cytosol. Overexpression of ABP7 and ABP9 resulted in dramatic inductions of ABA-induced gene expression in diverse wildtype and mutant Arabidopsis. The ABP7- and ABP9-mediated stimulation of ABA responses was antagonized by overexpression of ABI1 and ABI2. Arabidopsis thaliana with ectopic expression of ABP9 displayed ABA hypersensitivity in the root growth response, seed germination, and stomatal closure. The protein phosphatase assays demonstrated that ABP9 could inhibit the PP2C activities of ABI1 and ABI2 in an ABA-dependent manner. Die Proteinphosphatasen 2C ABI1 und ABI2 sind negative Schlüsselregulatoren der Signaltransduktion vom Pflanzenhormon Abscisinsäure (ABA). Im Rahmen dieser Arbeit erfolgte die Charakterisierung von ABP7 und ABP9, die als Interaktionspartner von ABI2 im Hefe-Zwei-Hybrid-System identifiziert wurden. In Arabidopsis-Protoplasten wirkten ABP7 und ABP9 auf sowohl ABI1 als auch ABI2 im Kern und im Zytosol ein. Im transienten Expressionssystem zeigte die Überexpression von ABP7 und ABP9 in Protoplasten eine drastische Induktion. Die ABP7- und ABP9-vermittelte Stimulierung der ABA-Antwort wird durch Ko-Expression von ABI1 und ABI2 antagonisiert. Arabidopsis thaliana mit ektopischer Expression von ABP9 zeigte eine ABA-Hypersentitivität in den Tests des Wurzelwachstums, der Samenkeimung und der Schließung der Stomata. ABP9 konnte die Proteinphosphataseaktivität von ABI1 und ABI2 auf eine ABA-abhängige Art und Weise blockieren.

Keywords

Biowissenschaften, Biologie, ddc: ddc:570

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
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