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Thesis . 2021
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The Use of Intestinal Organoids to Study Colorectal Cancer in Pigs

descriptionPublicationkeyboard_double_arrow_right Thesis 22 Nov 2021 English Publisher:Technical University of Munich
Authors: Grodziecki, Alessandro;

The Use of Intestinal Organoids to Study Colorectal Cancer in Pigs

Abstract

Colorectal cancer (CRC) is a major cause of cancer related deaths worldwide. Pigs genetically predisposed to CRC model early stages of the human disease but further mutations are required to recapitulate advanced disease. Using CRISPR/Cas9 multiplexing, tumour suppressors were inactivated in colonic organoids from these pigs. Additionally, we activated a silent KRAS-G12D allele. Transformed organoids exhibited hallmarks of cancer cells and will be used for autologous transplantation. Darmkrebs (DK) ist eine Haupttodesursache bei Krebserkrankungen weltweit. Schweine mit einer genetischen Prädisposition für DK modellieren frühe Stadien der Erkrankung, aber weitere Mutationen sind nötig, um Folgestadien abzubilden. Wir nutzten CRISPR/Cas9-Multiplexing, um Tumorsuppressorgene in Darmorganoiden dieser Schweine zu inaktiviert. Zusätzlich aktivierten wir ein latentes KRAS-G12D Allel. Transformierte Organoide ähneln Krebszellen und werden zur autologen Transplantation eingesetzt.

Keywords

Biowissenschaften, Biologie, Landwirtschaft, ddc: ddc:570, ddc: ddc:630

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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Cancer Research