Since conserved viral proteins of influenza virus, such as nucleoprotein (NP) and matrix 1 protein, are the main targets for virus-specific CD8+ cytotoxic T-lymphocytes (CTLs), we hypothesized that introduction of the NP gene of wild-type virus into the genome of vaccine reassortants could lead to better immunogenicity and afford better protection. This paper describes in vitro and in vivo preclinical studies of two new reassortants of pandemic H1N1 live attenuated influenza vaccine (LAIV) candidates. One had the hemagglutinin (HA) and neuraminidase (NA) genes from A/South Africa/3626/2013 H1N1 wild-type virus on the A/Leningrad/134/17/57 master donor virus backbone (6: 2 formulation) while the second had the HA, NA, and NP genes of the wild-type virus on the same backbone (5: 3 formulation). Although both LAIVs induced similar antibody immune responses, the 5: 3 LAIV provoked greater production of virus-specific CTLs than the 6: 2 variant. Furthermore, the 5: 3 LAIV-induced CTLs had higher in vivo cytotoxic activity, compared to 6: 2 LAIV. Finally, the 5: 3 LAIV candidate afforded greater protection against infection and severe illness than the 6: 2 LAIV. Inclusion in LAIV of the NP gene from wild-type influenza virus is a new approach to inducing cross-reactive cell-mediated immune responses and cross protection against pandemic influenza. © 2017 Andrey Rekstin et al.