Purpose: Comeal epithelial wound closure rates in rabbit eyes were compared after topical applications of moxifloxacin 0.5% ophthalmic solution, gatifloxacin 0.3% ophthalmic solution, and BSS Sterile Irrigating Solution. Methods: Thirty rabbits were divided into 3 randomization groups of 10 rabbits each. Animals in each randomization group were predosed 4 times a day with a different test article in each eye for 1 day before surgery. Group 1 animals received BSS in 1 eye and moxifloxacin in the other eye; group 2 animals received BSS in 1 eye and gatifloxacin in the other eye; and group 3 animals received moxifloxacin in 1 eye and gatifloxacin in the other eye, resulting in each test article's being administered to 20 eyes. Rabbits underwent a bilateral procedure to remove the full thickness of the central comeal epithelium within a 5-mm trephine mark. After wounding, the eyes were dosed 4 times a day with the same respective predose test articles, and epithelial wound closure was recorded using slit-lamp photography. The data were analyzed to determine the rate of wound closure. Results: The mean wound radius closure rate was 71.1 ± 11.7 μm/h for BSS-treated eyes, 67.6 ± 7.1 μm/h for moxifloxacin-treated eyes, and 65.9 ± 8.0 μm/h for gatifloxacin-treated eyes. Conclusions: The relative order of wound healing rates was BSS > moxifloxacin > gatifloxacin; however, differences between treatment groups were not considered significant.