Regulation of NMDA Receptor Transport: A KIF17–Cargo Binding/Releasing Underlies Synaptic Plasticity and MemoryIn Vivo
Copyright policy )Regulation of NMDA Receptor Transport: A KIF17–Cargo Binding/Releasing Underlies Synaptic Plasticity and MemoryIn Vivo
Regulation of NMDA receptor trafficking is crucial to modulate neuronal communication. Ca2+/calmodulin-dependent protein kinase phosphorylates the tail domain of KIF17, a member of the kinesin superfamily, to control NMDA receptor subunit 2B (GluN2B) transport by changing the KIF17–cargo interactionin vitro. However, the mechanisms of regulation of GluN2B transportin vivoand its physiological significance are unknown. We generated transgenic mice carrying wild-type KIF17 (TgS), or KIF17 with S1029A (TgA) or S1029D (TgD)phosphomimicmutations inkif17−/−background.TgA/kif17−/−andTgD/kif17−/−mice exhibited reductions in synaptic NMDA receptors because of their inability to load/unload GluN2B onto/from KIF17, leading to impaired neuronal plasticity, CREB activation, and spatial memory. Expression of GFP-KIF17 inTgS/kif17−/−mouse neurons rescued the synaptic and behavioral defects ofkif17−/−mice. These results suggest that phosphorylation-based regulation of NMDA receptor transport is critical for learning and memoryin vivo.
- University of Tokyo Japan
- King Abdulaziz University Saudi Arabia
Male, Analysis of Variance, Green Fluorescent Proteins, Long-Term Potentiation, Biophysics, Excitatory Postsynaptic Potentials, Kinesins, In Vitro Techniques, CREB-Binding Protein, Hippocampus, Electric Stimulation, DNA-Binding Proteins, Animals, Immunoprecipitation, Biotinylation, Cycloheximide, Maze Learning, Disks Large Homolog 4 Protein, Guanylate Kinases, Cells, Cultured
Male, Analysis of Variance, Green Fluorescent Proteins, Long-Term Potentiation, Biophysics, Excitatory Postsynaptic Potentials, Kinesins, In Vitro Techniques, CREB-Binding Protein, Hippocampus, Electric Stimulation, DNA-Binding Proteins, Animals, Immunoprecipitation, Biotinylation, Cycloheximide, Maze Learning, Disks Large Homolog 4 Protein, Guanylate Kinases, Cells, Cultured
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).51 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!