Mutational Analysis of the Yeast TRAPP Subunit Trs20p Identifies Roles in Endocytic Recycling and Sporulation
Copyright policy )Mutational Analysis of the Yeast TRAPP Subunit Trs20p Identifies Roles in Endocytic Recycling and Sporulation
Trs20p is a subunit of the evolutionarily conserved TRAPP (TRAnsport Protein Particle) complex that mediates various aspects of membrane trafficking. Three TRAPP complexes have been identified in yeast with roles in ER-to-Golgi trafficking, post-Golgi and endosomal-to-Golgi transport and in autophagy. The role of Trs20p, which is essential for viability and a component of all three complexes, and how it might function within each TRAPP complex, has not been clarified to date. To begin to address the role of Trs20p we generated different mutants by random mutagenesis but, surprisingly, no defects were observed in diverse anterograde transport pathways or general secretion in Trs20 temperature-sensitive mutants. Instead, mutation of Trs20 led to defects in endocytic recycling and a block in sporulation/meiosis. The phenotypes of different mutants appear to be separable suggesting that the mutations affect the function of Trs20 in different TRAPP complexes.
Spores, Genetics and Molecular Biology (all), Models, Molecular, 570, Saccharomyces cerevisiae Proteins, Genotype, Science, DNA Mutational Analysis, Vesicular Transport Proteins, Saccharomyces cerevisiae, Trs20/sedlin, Biochemistry, Models, Golgi, Settore BIO/10 - BIOCHIMICA, Medicine (all), Q, R, 500, Molecular, Spores, Fungal, Yeast, TRAPP, Endocytosis, Meiosis, Protein Subunits, Fungal, Phenotype, Agricultural and Biological Sciences (all), Mutation, Medicine, intracellular traffic, DNA Mutational Analysis; Endocytosis; Genotype; Meiosis; Models, Molecular; Phenotype; Protein Subunits; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Spores, Fungal; Vesicular Transport Proteins; Mutation; Agricultural and Biological Sciences (all); Biochemistry, Genetics and Molecular Biology (all); Medicine (all), Research Article
Spores, Genetics and Molecular Biology (all), Models, Molecular, 570, Saccharomyces cerevisiae Proteins, Genotype, Science, DNA Mutational Analysis, Vesicular Transport Proteins, Saccharomyces cerevisiae, Trs20/sedlin, Biochemistry, Models, Golgi, Settore BIO/10 - BIOCHIMICA, Medicine (all), Q, R, 500, Molecular, Spores, Fungal, Yeast, TRAPP, Endocytosis, Meiosis, Protein Subunits, Fungal, Phenotype, Agricultural and Biological Sciences (all), Mutation, Medicine, intracellular traffic, DNA Mutational Analysis; Endocytosis; Genotype; Meiosis; Models, Molecular; Phenotype; Protein Subunits; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Spores, Fungal; Vesicular Transport Proteins; Mutation; Agricultural and Biological Sciences (all); Biochemistry, Genetics and Molecular Biology (all); Medicine (all), Research Article
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