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Umbilical cord blood-derived dendritic cells infected by adenovirus for SP17 expression induce antigen-specific cytotoxic T cells against NSCLC cells

descriptionPublicationkeyboard_double_arrow_right Article 01 Nov 2015 English Publisher:Elsevier BVJournal:Cellular Immunology, volume 298, pages 18-24 (issn: 0008-8749,

Authors: Shenyi Jiang; Jichun Yang; Xuemei Ren; Yang Liu; Youhong Jiang; Yanling Chen; Xin Tian; +1 Authors

doi: 10.1016/j.cellimm.2015.08.004

pmid: 26300426

Umbilical cord blood-derived dendritic cells infected by adenovirus for SP17 expression induce antigen-specific cytotoxic T cells against NSCLC cells

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Abstract

Sperm protein 17 (SP17), a cancer/testis antigen, is expressed by non-small cell lung cancer (NSCLC). This study examined whether dendritic cells (DC) from human umbilical cord blood (UCB) could be induced for SP17 expression and induce antigen-specific CD8(+) cytotoxic T lymphocytes (CTLs) against NSCLC in vitro. We generated recombinant adenovirus of Ad-SP17 and control Ad-null. Infection with Ad-SP17, but not control, induced higher levels of SP17 expression in UCB-derived DC-Ad-SP17. Infection with Ad-SP17 significantly increased the frequency of CD80(+), CD83(+), CD86(+), and HLA-DR(+) DC that produced higher levels of IL-12, but lower IL-10. Co-culture of DC-Ad-SP17 with autologous UCB lymphocytes induced high frequency of IFNγ(+) CD8(+) CTLs, which had selective cytotoxicity against SP17(+) lung cancer CRL-5922 cells in a HLA-I restrictive manner. Thus, UCB-derived DC modulated for SP17 expression induced antigen-specific anti-tumor immunity against SP17(+) NSCLC, and SP17 may be a valuable target for development of immunotherapy against SP17(+) NSCLC.

Related Organizations

China Medical University
China (People's Republic of)
First Hospital of China Medical University
China (People's Republic of)

Keywords

Cytotoxicity, Immunologic, Lung Neoplasms, Membrane Proteins, Dendritic Cells, Fetal Blood, Coculture Techniques, Interleukin-12 Subunit p35, Adenoviridae, Interleukin-10, Interferon-gamma, Antigens, Neoplasm, Carcinoma, Non-Small-Cell Lung, Cell Line, Tumor, Antigens, Surface, Humans, Calmodulin-Binding Proteins, Immunotherapy, Carrier Proteins, T-Lymphocytes, Cytotoxic

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Sperm protein 17
2017IsRelatedTo
SPA17 protein
2017IsRelatedTo
Sperm surface protein Sp17
2017IsRelatedTo

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