Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR46 azole resistance gene
Copyright policy )Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR46 azole resistance gene
AbstractInfections due to triazole-resistant Aspergillus fumigatus are increasingly reported worldwide and are associated with treatment failure and mortality. The principal class of azole-resistant isolates is characterized by tandem repeats of 34 bp or 46 bp within the promoter region of the cyp51A gene. Loop-mediated isothermal amplification (LAMP) is a widely used nucleic acid amplification system that is fast and specific. Here we describe a LAMP assay method to detect the 46 bp tandem repeat insertion in the cyp51A gene promoter region based on novel LAMP primer sets. It also differentiated strains with TR46 tandem repeats from those with TR34 tandem repeats. These results showed this TR46-LAMP method is specific, rapid, and provides crucial insights to develop novel antifungal therapeutic strategies against severe fungal infections due to A. fumigatus with TR46 tandem repeats.
- University of Tsukuba Japan
- Chiba University Japan
- University of Nagasaki Japan
- State University of Campinas Brazil
Azoles, Antifungal Agents, Science, Aspergillus fumigatus, Q, R, Article, Fungal Proteins, Cytochrome P-450 Enzyme System, Molecular Diagnostic Techniques, Drug Resistance, Fungal, Tandem Repeat Sequences, Medicine, Promoter Regions, Genetic, Nucleic Acid Amplification Techniques, DNA Primers
Azoles, Antifungal Agents, Science, Aspergillus fumigatus, Q, R, Article, Fungal Proteins, Cytochrome P-450 Enzyme System, Molecular Diagnostic Techniques, Drug Resistance, Fungal, Tandem Repeat Sequences, Medicine, Promoter Regions, Genetic, Nucleic Acid Amplification Techniques, DNA Primers
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