Determining exon connectivity in complex mRNAs by nanopore sequencing
Copyright policy )Determining exon connectivity in complex mRNAs by nanopore sequencing
AbstractThough powerful, short-read high throughput RNA sequencing is limited in its ability to directly measure exon connectivity in mRNAs containing multiple alternative exons located farther apart than the maximum read lengths. Here, we use the Oxford Nanopore MinION™sequencer to identify 7,899 ‘full-length’ isoforms expressed from fourDrosophilagenes,Dscam1,MRP,Mhc, andRdl. These results demonstrate that nanopore sequencing can be used to deconvolute individual isoforms and that it has the potential to be an important method for comprehensive transcriptome characterization.
- University of Connecticut Health Center United States
Method, High-Throughput Nucleotide Sequencing, Exons, Myosins, Receptors, GABA-A, Nanopores, Animals, Drosophila Proteins, Protein Isoforms, ATP-Binding Cassette Transporters, Drosophila, RNA, Messenger, Transcriptome, Cell Adhesion Molecules, Neural Cell Adhesion Molecules
Method, High-Throughput Nucleotide Sequencing, Exons, Myosins, Receptors, GABA-A, Nanopores, Animals, Drosophila Proteins, Protein Isoforms, ATP-Binding Cassette Transporters, Drosophila, RNA, Messenger, Transcriptome, Cell Adhesion Molecules, Neural Cell Adhesion Molecules
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