AnkyrinG Is Required for Clustering of Voltage-gated Na Channels at Axon Initial Segments and for Normal Action Potential Firing
Copyright policy )AnkyrinG Is Required for Clustering of Voltage-gated Na Channels at Axon Initial Segments and for Normal Action Potential Firing
Voltage-gated sodium channels (NaCh) are colocalized with isoforms of the membrane-skeletal protein ankyrinG at axon initial segments, nodes of Ranvier, and postsynaptic folds of the mammalian neuromuscular junction. The role of ankyrinG in directing NaCh localization to axon initial segments was evaluated by region-specific knockout of ankyrinG in the mouse cerebellum. Mutant mice exhibited a progressive ataxia beginning around postnatal day P16 and subsequent loss of Purkinje neurons. In mutant mouse cerebella, NaCh were absent from axon initial segments of granule cell neurons, and Purkinje cells showed deficiencies in their ability to initiate action potentials and support rapid, repetitive firing. Neurofascin, a member of the L1CAM family of ankyrin-binding cell adhesion molecules, also exhibited impaired localization to initial segments of Purkinje cell neurons. These results demonstrate that ankyrinG is essential for clustering NaCh and neurofascin at axon initial segments and is required for physiological levels of sodium channel activity.
- University of Massachusetts Medical School United States
- Duke University Hospital United States
- Howard Hughes Medical Institute United States
- Duke University Medical Center United States
- University of Minnesota Morris United States
Ankyrins, Mice, Knockout, Base Sequence, Molecular Sequence Data, Action Potentials, Axons, Sodium Channels, Mice, Inbred C57BL, Mice, Purkinje Cells, Cerebellum, Nerve Degeneration, Animals, Ataxia, Amino Acid Sequence, Nerve Growth Factors, Oligonucleotide Probes, Cell Adhesion Molecules
Ankyrins, Mice, Knockout, Base Sequence, Molecular Sequence Data, Action Potentials, Axons, Sodium Channels, Mice, Inbred C57BL, Mice, Purkinje Cells, Cerebellum, Nerve Degeneration, Animals, Ataxia, Amino Acid Sequence, Nerve Growth Factors, Oligonucleotide Probes, Cell Adhesion Molecules
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