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Article . 2004
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The Journal of Chemical Physics
Article . 2004 . Peer-reviewed
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Rotational dynamics of colloidal spheres probed with fluorescence recovery after photobleaching

Authors: Lettinga, Pavlik; Koenderink, G.H.; Kuipers, B.WM.; Bessels, E.; Philipse, A.P.;

Rotational dynamics of colloidal spheres probed with fluorescence recovery after photobleaching

Abstract

We report a polarized fluorescence recovery after photobleaching (pFRAP) method to measure the rotational dynamics of fluorescent colloids over a wide dynamic range. The method is based on the polarization anisotropy in the fluorescence intensity, generated by bleaching of fluorescently labeled particles with an intense pulse of linearly polarized laser light. The rotational mobilities of the fluorescent particles can be extracted from the relaxation kinetics of the postbleach fluorescence polarization anisotropy. Our pFRAP setup has access to correlation times over a range of time scales from tens of microseconds to tens of seconds, and is highly sensitive, so very low concentrations of labeled particles can be probed. We present a detailed description of the theoretical background of pFRAP. The performance of the equipment is demonstrated for fluorescent colloidal silica spheres, dispersed in pure solvents as well as in fd-virus suspensions.

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Keywords

Models, Molecular, info:eu-repo/classification/ddc/540, Rotation, Colloids: chemistry, GLASS-TRANSITION, Molecular Conformation, SILICA SPHERES, Physics, Atomic, Molecular & Chemical, 09 Engineering, RESOLVED PHOSPHORESCENCE ANISOTROPY, Diffusion, Fluorescence Recovery After Photobleaching: methods, Colloids: analysis, DEPOLARIZATION, Biomechanics, Computer Simulation, Colloids, 40 Engineering, ACETYLCHOLINE-RECEPTORS, Science & Technology, 02 Physical Sciences, Chemical Physics, Chemistry, Physical, Physics, 34 Chemical sciences, DIFFUSION, Microspheres, J, TIME, Biomechanical Phenomena, Chemistry, Models, Chemical, POLARIZED FLUORESCENCE, Physical Sciences, Stress, Mechanical, 51 Physical sciences, 03 Chemical Sciences, TRACER SPHERES, O-TERPHENYL, Fluorescence Recovery After Photobleaching

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    influence
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
25
Average
Top 10%
Top 10%
Green
bronze