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Centromere protein-F (CENP-F) is mammalian kinetochore protein that was recently identified by an autoimmune serum (Rattner, J. B., A. Rao, M. J. Fritzler, D. W. Valencia, and T. J. Yen. Cell Motil. Cytoskeleton. 26:214-226). We report here the human cDNA sequence of CENP-F, along with its expression and localization patterns at different stages of the HeLa cell cycle. CENP-F is protein of the nuclear matrix that gradually accumulates during the cell cycle until it reaches peak levels in G2 and M phase cells and is rapidly degraded upon completion of mitosis. CENP-F is first detected at the prekinetochore complex during late G2, and is clearly detectable as paired foci that correspond to all the centromeres by prophase. During mitosis, CENP-F is associated with kinetochores from prometaphase until early anaphase and is then detected at the spindle midzone throughout the remainder of anaphase. By telophase, CENP-F is concentrated within the intracellular bridge at either side of the mid-body. The predicted structure of the 367-kD CENP-F protein consists of two 1,600-amino acid-long coil domains that flank a central flexible core. A putative P-loop nucleotide binding site (ADIPTGKT) is located within the globular carboxy terminus. The structural features deduced from our sequence studies and the spatial and temperal distribution of CENP-F revealed in our cytological and biochemical studies suggest that it may play a role in several mitotic events.
Cell Nucleus, G2 Phase, DNA, Complementary, Base Sequence, Chromosomal Proteins, Non-Histone, Microfilament Proteins, Fluorescent Antibody Technique, Mitosis, Antigens, Nuclear, Cross Reactions, Autoantigens, Cell Compartmentation, Epitopes, Humans, Amino Acid Sequence, Cloning, Molecular, Kinetochores, Cell Division, Autoantibodies, HeLa Cells
Cell Nucleus, G2 Phase, DNA, Complementary, Base Sequence, Chromosomal Proteins, Non-Histone, Microfilament Proteins, Fluorescent Antibody Technique, Mitosis, Antigens, Nuclear, Cross Reactions, Autoantigens, Cell Compartmentation, Epitopes, Humans, Amino Acid Sequence, Cloning, Molecular, Kinetochores, Cell Division, Autoantibodies, HeLa Cells
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