Coupled regulations of enzymatic activity and structure formation of aldehyde dehydrogenase Ald4p
Copyright policy )Coupled regulations of enzymatic activity and structure formation of aldehyde dehydrogenase Ald4p
Previously, we have developed an extramitochondrial assembly system, where mitochondrial targeting signal (MTS) can be removed from a given mitochondrial enzyme, which could be used to characterize the regulatory factors involved in enzyme assembly/disassembly in vivo. Here, we demonstrate that addition of exogenous acetaldehyde can shortly induce the supramolecular assembly of MTS-deleted aldehyde dehydrogenase Ald4p in yeast cytoplasm. Also, by using PCR-based modification of the yeast genome, cytoplasmically targeted Ald4p cannot polymerize into long filaments when key functional amino acid residues are substituted, as shown by N192D, S269A, E290K, and C324A mutations. This study has confirmed that extramitochondrial assembly could be a powerful external system for studying mitochondrial enzyme assembly and its regulatory factors, outside the mitochondria. And we propose that mitochondrial enzyme assembly/disassembly is coupled to the regulation of a given mitochondrial enzyme activity.
- Mahidol University Thailand
Models, Molecular, Cytoplasm, Cell biology, QH301-705.5, Protein Conformation, Science, Recombinant Fusion Proteins, Materials Science, Fluorescent Antibody Technique, Acetaldehyde, Saccharomyces cerevisiae, yeast, Biochemistry, Enzyme Regulation, Structure-Activity Relationship, aldehyde dehydrogenase, Catalytic Domain, Biochemistry, Genetics and Molecular Biology, Materials Chemistry, Amino Acid Sequence, Biology (General), Mitochondrion, Molecular Biology, Biology, Binding Sites, Macromolecular Crystallography Techniques, Ethanol, Q, Genomic Expression and Function in Yeast Organism, Life Sciences, Aldehyde dehydrogenase, Aldehyde Dehydrogenase, Yeast, Mitochondria, Nucleotide Metabolism and Enzyme Regulation, Enzyme Activation, Enzyme, Physical Sciences, extramitochondrial assembly, Research Article, Protein Binding
Models, Molecular, Cytoplasm, Cell biology, QH301-705.5, Protein Conformation, Science, Recombinant Fusion Proteins, Materials Science, Fluorescent Antibody Technique, Acetaldehyde, Saccharomyces cerevisiae, yeast, Biochemistry, Enzyme Regulation, Structure-Activity Relationship, aldehyde dehydrogenase, Catalytic Domain, Biochemistry, Genetics and Molecular Biology, Materials Chemistry, Amino Acid Sequence, Biology (General), Mitochondrion, Molecular Biology, Biology, Binding Sites, Macromolecular Crystallography Techniques, Ethanol, Q, Genomic Expression and Function in Yeast Organism, Life Sciences, Aldehyde dehydrogenase, Aldehyde Dehydrogenase, Yeast, Mitochondria, Nucleotide Metabolism and Enzyme Regulation, Enzyme Activation, Enzyme, Physical Sciences, extramitochondrial assembly, Research Article, Protein Binding
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