Retaking routes after a period of time usually occurs in an environment which has suffered from spatial configuration modifications. Thus, the original visual stimuli that allowed us to establish cognitive mapping using an allocentric strategy during the acquisition phase may not remain physically identical at the time of retrieval. However, in the standard experimental paradigms the cues are typically maintained constant. In this study, we explored memory retrieval with spatial modifications from learning in the Morris Water Maze. We trained rats on a reference memory protocol with five cues placed on black curtains that surrounded the pool, and seven days later, we tested memory retrieval under different conditions: maintenance of the five cues, removal of two and four of them, and the addition of three extra ones. Under full-cue and partial cue-conditions, rats showed successful memory retrieval, whereas adding extra cues resulted in impaired retrieval. Furthermore, we assessed brain oxidative metabolism through cytochrome c oxidase (CCO) histochemistry and found that, under full- and partial-cue conditions, there is an enhancement of the hippocampal, prefrontal, retrosplenial, parietal, and rhinal cortex metabolism. Rats that failed to retrieve spatial information in the extra cues condition showed similar or lower CCO activity than controls across many limbic areas. It is suggested that the presence of a partial portion of visual stimuli from learning makes it possible to reactivate the entire memory trace, but extra spatial information hinders retrieval, making it difficult to disengage the novel information from the older knowledge and establish a contextual generalization.