Global expression analysis of gene regulatory pathways during endocrine pancreatic development
Copyright policy )Global expression analysis of gene regulatory pathways during endocrine pancreatic development
To define genetic pathways that regulate development of the endocrine pancreas, we generated transcriptional profiles of enriched cells isolated from four biologically significant stages of endocrine pancreas development:endoderm before pancreas specification, early pancreatic progenitor cells,endocrine progenitor cells and adult islets of Langerhans. These analyses implicate new signaling pathways in endocrine pancreas development, and identified sets of known and novel genes that are temporally regulated, as well as genes that spatially define developing endocrine cells from their neighbors. The differential expression of several genes from each time point was verified by RT-PCR and in situ hybridization. Moreover, we present preliminary functional evidence suggesting that one transcription factor encoding gene (Myt1), which was identified in our screen, is expressed in endocrine progenitors and may regulate α, β andδ cell development. In addition to identifying new genes that regulate endocrine cell fate, this global gene expression analysis has uncovered informative biological trends that occur during endocrine differentiation.
- Massachusetts General Hospital United States
- Harvard University United States
Mice, Inbred ICR, Transcription, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Green Fluorescent Proteins, Gene Expression Regulation, Developmental, Cell Differentiation, Mice, Transgenic, Protein Serine-Threonine Kinases, Protein-Tyrosine Kinases, DNA-Binding Proteins, Embryonic and Fetal Development, Islets of Langerhans, Luminescent Proteins, Mice, Pregnancy, Animals, Female, In Situ Hybridization, Transcription Factors
Mice, Inbred ICR, Transcription, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Green Fluorescent Proteins, Gene Expression Regulation, Developmental, Cell Differentiation, Mice, Transgenic, Protein Serine-Threonine Kinases, Protein-Tyrosine Kinases, DNA-Binding Proteins, Embryonic and Fetal Development, Islets of Langerhans, Luminescent Proteins, Mice, Pregnancy, Animals, Female, In Situ Hybridization, Transcription Factors
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