Design of a polyepitope construct for the induction of HLA-A0201-restricted HIV 1-specific CTL responses usingHLA-A*0201 transgenic,H-2 class I KO mice
Copyright policy )Design of a polyepitope construct for the induction of HLA-A0201-restricted HIV 1-specific CTL responses usingHLA-A*0201 transgenic,H-2 class I KO mice
HLA-A*0201 transgenic, H-2D(b)/mouse beta2-microglobulin double-knockout mice were used to compare and optimize the immunogenic potential of 17HIV 1-derived,HLA-A0201-restricted epitopic peptides. A tyrosine substitution in position 1 of the epitopic peptides, which increases both their affinity for and their HLA-A0201 molecule stabilizing capacity, was introduced in a significant proportion, having verified that such modifications enhance their immunogenicity in respect of their natural antigenicity. Based on these results, a 13-polyepitope construct was inserted in the pre-S2 segment of the hepatitis B middle glycoprotein and used for DNA immunization. Long-lasting CTL responses against most of the inserted epitopes could be elicited simultaneously in a single animal with cross-recognition in several cases of their most common natural variants.
- Centre national de la recherche scientifique France
- University of Queensland Australia
- Institut Pasteur France
- University of Queensland Australia
- QIMR Berghofer Medical Research Institute Australia
570, Immunology, Molecular Sequence Data, Virus Type-1 Infection, 730102 Immune system and allergy, Major Histocompatibility Complex, Epitopes, Mice, C1, Cell Responses, 616, Vaccines, DNA, Animals, Viremia, Amino Acid Sequence, H-2 Class Iko Mouse, Histocompatibility Antigen H-2D, Hiv 1 Vaccine, AIDS Vaccines, Mice, Knockout, Base Sequence, HLA-A Antigens, Cytotoxic T-lymphocytes, H-2 Antigens, Reverse-transcriptase, Ctl, Peptide Binding, 320299 Immunology not elsewhere classified, Hla Class I Transgenic Mouse, Disease Progression, HIV-1, Tyrosine, Immunization, Immune-responses, T-Lymphocytes, Cytotoxic
570, Immunology, Molecular Sequence Data, Virus Type-1 Infection, 730102 Immune system and allergy, Major Histocompatibility Complex, Epitopes, Mice, C1, Cell Responses, 616, Vaccines, DNA, Animals, Viremia, Amino Acid Sequence, H-2 Class Iko Mouse, Histocompatibility Antigen H-2D, Hiv 1 Vaccine, AIDS Vaccines, Mice, Knockout, Base Sequence, HLA-A Antigens, Cytotoxic T-lymphocytes, H-2 Antigens, Reverse-transcriptase, Ctl, Peptide Binding, 320299 Immunology not elsewhere classified, Hla Class I Transgenic Mouse, Disease Progression, HIV-1, Tyrosine, Immunization, Immune-responses, T-Lymphocytes, Cytotoxic
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