ABSTRACT During a 3-year study period, 32,741 urine samples were analyzed for the presence of members of the Staphylococcus sciuri group ( S. sciuri , S. lentus , and S. vitulinus ), and 13 isolates were identified. They presented 0.79% of the total number of coagulase-negative staphylococci isolated. One case of symptomatic urinary tract infection and five possible cases of asymptomatic bacteriuria caused by these bacteria were established. It is noteworthy, however, that over 50% of the isolates originated from hospitalized patients.