Chicken ovalbumin upstream promoter-transcription factor I represses the transcriptional activity of the human muscle glycogen phosphorylase promoter in C2C12 cells
Copyright policy )Chicken ovalbumin upstream promoter-transcription factor I represses the transcriptional activity of the human muscle glycogen phosphorylase promoter in C2C12 cells
The responsiveness of the 1.13 kb proximal human muscle glycogen phosphorylase (MGP) gene promoter to the chicken ovalbumin upstream promoter-transcription factor (COUP-TF) repressor, known to be ablated during muscle cell differentiation, was examined. Constitutive expression of COUP-TFI repressed the activity of the promoter in C2C12 muscle cells and sequential deletion analysis mapped the sensitive region between nucleotides -362 and -185, which included a putative consensus COUP-TF binding half-site at -198/-193. Mutation of this site abolished transcriptional response to COUP-TFI of the -362 construct. A -209/-180 probe bound in vitro to COUP-TFI and to protein extracts from proliferating but not fusing myoblasts. Thus, COUP-TF may be involved in repression of the human MGP gene promoter at the myoblast stage.
- University of Barcelona Spain
Cell Nucleus, Receptors, Steroid, Binding Sites, COUP Transcription Factor I, Models, Genetic, Transcription, Genetic, Muscles, Glycogen Phosphorylase, Cell Differentiation, Cell Line, DNA-Binding Proteins, Mice, Animals, Humans, Promoter Regions, Genetic, Cell Division, Gene Deletion, DNA Primers, Protein Binding, Transcription Factors
Cell Nucleus, Receptors, Steroid, Binding Sites, COUP Transcription Factor I, Models, Genetic, Transcription, Genetic, Muscles, Glycogen Phosphorylase, Cell Differentiation, Cell Line, DNA-Binding Proteins, Mice, Animals, Humans, Promoter Regions, Genetic, Cell Division, Gene Deletion, DNA Primers, Protein Binding, Transcription Factors
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).5 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!