<script type="text/javascript">
<!--
document.write('<div id="oa_widget"></div>');
document.write('<script type="text/javascript" src="https://www.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=undefined&type=result"></script>');
-->
</script>

COPY SCRIPT

For further information contact us at helpdesk@openaire.eu

Cyclin A2 and its associated kinase activity are required for optimal induction of progesterone receptor target genes in breast cancer cells

descriptionPublicationkeyboard_double_arrow_right Article 01 Oct 2014 Australia English Publisher:Elsevier BVJournal:The Journal of Steroid Biochemistry and Molecular Biology, volume 144, pages 471-482 (issn: 0960-0760,

Authors: Moore, N.; Edwards, D.; Weigel, N.;

doi: 10.1016/j.jsbmb.2014.09.009

pmid: 25220500

pmc: PMC4201666

handle: 2440/90926

Cyclin A2 and its associated kinase activity are required for optimal induction of progesterone receptor target genes in breast cancer cells

- Summary
- Subjects
- Related research
  (7)
- Metrics

Abstract

A role for the cell cycle protein cyclin A2 in regulating progesterone receptor (PR) activity is emerging. This study investigates the role of cyclin A2 in regulating endogenous PR activity in T47D breast cancer cells by depleting cyclin A2 expression and measuring PR target genes using q-RT-PCR. Targets examined included genes induced by the PR-B isoform more strongly than PR-A (SGK1, FKBP5), a gene induced predominantly by PR-A (HEF1), genes induced via PR tethering to other transcription factors (p21, p27), a gene induced in part via extra-nuclear PR signaling mechanisms (cyclin D1) and PR-repressed genes (DST, IL1R1). Progestin induction of target genes was reduced following cyclin A2 depletion. However, cyclin A2 depletion did not diminish progestin target gene repression. Furthermore, inhibition of the associated Cdk2 kinase activity of cyclin A2 also reduced progestin induction of target genes, while Cdk2 enhanced the interaction between PR and cyclin A2. These results demonstrate that cyclin A2 and its associated kinase activity are important for progestin-induced activation of endogenous PR target genes in breast cancer cells.

Country

Australia

Related Organizations

University of Adelaide
Australia
Baylor College of Medicine
United States

Keywords

Cyclin dependent kinase 2, Cyclin-Dependent Kinase 2, Breast Neoplasms, Progesterone receptor, Purines, Cell Line, Tumor, Roscovitine, Humans, Female, RNA, Messenger, Receptors, Progesterone, Cyclin A2

7 Research products, page 1 of 1

Cyclin-A2
2017IsRelatedTo
Cyclin-dependent kinase 2
2017IsRelatedTo
Cyclin-dependent kinase 2, isoform CRA_a
2017IsRelatedTo
Cyclin-dependent kinase 2, isoform CRA_c
2017IsRelatedTo
Cyclin-dependent kinase 2
2017IsRelatedTo
Cyclin-dependent kinase 2, isoform CRA_b
2017IsRelatedTo
cDNA FLJ54979, highly similar to Homo sapiens cyclin-dependent kinase 2 (CDK2), transcript variant 2
2017IsRelatedTo

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	16
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Top 10%
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average