Purpose: To study the diagnostic yield and safety aspects of large needle core biopsy of pathologic lesions in the axillary region using a semi‐automatic biopsy gun. Material and Methods: A series of 21 consecutive large needle core (14G, 2.1 mm) biopsies of the axilla was evaluated. The biopsies were performed under ultrasound guidance with one ( n=13) or two ( n=8) needle passes. The results were correlated to the clinical, mammographic, ultrasound and surgical histopathologic findings. Results: In 18 patients, specimens large enough for diagnosis were retrieved. In two patients the specimens were insufficient and non‐diagnostic. In one patient with a non‐malignant yield the result was false‐negative. No neural or vascular injury occurred. Conclusion: The procedure might be safely employed in the diagnosis of ultrasonographically demonstrable lesions of the axilla, whenever specimens larger than those obtainable with a fine needle are desired.