Genetic Mapping of 262 Loci Derived from Expressed Sequences in a Murine Interspecific Cross Using Single-Strand Conformational Polymorphism Analysis
Copyright policy )Genetic Mapping of 262 Loci Derived from Expressed Sequences in a Murine Interspecific Cross Using Single-Strand Conformational Polymorphism Analysis
We have demonstrated previously that noncoding sequences of genes are a robust source of polymorphisms between mouse species when tested using single-strand conformation polymorphism (SSCP) analysis, and that these polymorphisms are useful for genetic mapping. In this report we demonstrate that presumptive 3′-untranslated region sequence obtained from expressed sequence tags (ESTs) can be analyzed in a similar fashion, and we have used this approach to map 262 loci using an interspecific backcross. These results demonstrate SSCP analysis of genes or ESTs is a simple and efficient means for the genetic localization of transcribed sequences, and is furthermore an approach that is applicable to any system for which there is sufficient sequence polymorphism.
- Harvard University United States
- Brigham and Women's Faulkner Hospital United States
- University of Maryland School of Medicine United States
- Jackson Laboratory United States
- University of Colorado Cancer Center United States
Genetic Markers, Mice, Inbred C3H, Transcription, Genetic, DNA Mutational Analysis, Chromosome Mapping, Mice, Inbred Strains, Mice, Inbred C57BL, Muridae, Mice, Mice, Inbred AKR, Mice, Inbred DBA, Animals, Crosses, Genetic, Polymorphism, Single-Stranded Conformational, Gene Library, Sequence Tagged Sites
Genetic Markers, Mice, Inbred C3H, Transcription, Genetic, DNA Mutational Analysis, Chromosome Mapping, Mice, Inbred Strains, Mice, Inbred C57BL, Muridae, Mice, Mice, Inbred AKR, Mice, Inbred DBA, Animals, Crosses, Genetic, Polymorphism, Single-Stranded Conformational, Gene Library, Sequence Tagged Sites
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).43 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!