Matrix metalloproteinase‐2, caveolins, focal adhesion kinase and c‐Kit in cells of the mouse myocardium
Copyright policy )Matrix metalloproteinase‐2, caveolins, focal adhesion kinase and c‐Kit in cells of the mouse myocardium
AbstractMatrix metalloproteinase‐2 (MMP‐2) may play roles at intracellular and extracellular sites of the heart in ischaemia/reperfusion injury. Caveolins (Cav‐1, ‐2 and ‐3) are lipid raft proteins which play roles in cell sig‐nalling. This study examined, using immunohistochemistry and two photon confocal microscopy, if MMP‐2 and caveolins co‐localize at the plasma membrane of cardiac cells: cardiomyocytes (CM), fibroblasts (FB) and capillary endothelial cells (CEC) in the left ventricle (LV) of the Cav‐1+/+ and Cav‐1−/− mouse heart. In Cav‐1+/+ mouse LV MMP‐2 and Cav‐1 co‐localized at CM plasma membranes, and at multiple locations in FB and CEC. MMP‐2 co‐localized with Cav‐2 only at CEC. MMP‐2 co‐localized with Cav‐3 at CM plasma membranes and Z‐lines, and partially at FB and CEC. In Cav‐1−/− LV Cav‐1 and MMP‐2 were absent or reduced everywhere. Cav‐2 appeared at CEC despite the absence of Cav‐1. Cav‐3 appeared at CM plasma membranes and Z‐lines, FB and CEC. Also, FAK in FB and c‐Kit in interstitial Cajal‐like cells (ICLC) were completely absent. By transmission electron microscopy in Cav‐1+/+, regular size caveolae (Cav) were at CEC, irregular size Cav were at CM and a few were at FB. In Cav‐1−/− there were few Cav at CM and FB and some at CEC. To conclude, MMP‐2 is closely associated with caveolins at FB and CEC as well as at CM. Also, MMP‐2 is closely associated with FAK at FB and c‐Kit at ICLC. Thus, Cav‐1 expression is not necessary for Cav‐2 expression. Cav‐3 or Cav‐3 with Cav‐2 has the capability to make Cav.
- University of Alberta Canada
Male, Caveolin 3, Caveolin 2, Caveolin 1, Cardiomyocyte, Caveolins, Mice, Matrix Metalloproteinase‐2, Von Willebrand Factor, von Willebrand Factor, Animals, Protein Isoforms, Myocytes, Cardiac, In Focus, Discoidin Domain Receptors, Myocardium, Receptor Protein-Tyrosine Kinases, Capillary Endothelial Cell, Fibroblasts, Focal Adhesion Kinase, Protein Transport, Proto-Oncogene Proteins c-kit, C‐Kit, Focal Adhesion Protein-Tyrosine Kinases, Receptors, Mitogen, Matrix Metalloproteinase 2, Fibroblast, Endothelium, Vascular, Interstitial Cajal‐Like Cell
Male, Caveolin 3, Caveolin 2, Caveolin 1, Cardiomyocyte, Caveolins, Mice, Matrix Metalloproteinase‐2, Von Willebrand Factor, von Willebrand Factor, Animals, Protein Isoforms, Myocytes, Cardiac, In Focus, Discoidin Domain Receptors, Myocardium, Receptor Protein-Tyrosine Kinases, Capillary Endothelial Cell, Fibroblasts, Focal Adhesion Kinase, Protein Transport, Proto-Oncogene Proteins c-kit, C‐Kit, Focal Adhesion Protein-Tyrosine Kinases, Receptors, Mitogen, Matrix Metalloproteinase 2, Fibroblast, Endothelium, Vascular, Interstitial Cajal‐Like Cell
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).36 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!