Identification of anArabidopsismitochondrial succinate–fumarate translocator
Copyright policy )Identification of anArabidopsismitochondrial succinate–fumarate translocator
Complementation of a yeastacr1mutant carrying a deletion of the succinate/fumarate carrier gene enabled functional identification of a mitochondrial succinate translocator fromArabidopsis thaliana(AtmSFC1). Thus complementation of yeast mutants is applicable also for identification and characterization of organellar transporters. Reverse transcription polymerase chain reaction and promoter‐GUS fusion showed expression of AtmSFC1 in 2 day old dark grown seedlings, which declined in cotyledons during further development, consistent with a role in export of fumarate for gluconeogenesis during lipid mobilization at early germination ofArabidopsisseeds. In mature plants, expression was found in developing and germinating pollen, suggesting a role in ethanolic fermentation.
- University of Cologne Germany
- Universtity of Cologne Germany
Saccharomyces cerevisiae Proteins, Arabidopsis thaliana, Recombinant Fusion Proteins, Molecular Sequence Data, Arabidopsis, Anaplerotic reaction, Mitochondrial Proteins, Fumarates, Gene Expression Regulation, Plant, Amino Acid Sequence, Cloning, Molecular, Promoter Regions, Genetic, Phylogeny, Arabidopsis Proteins, Genetic Complementation Test, Gluconeogenesis, Mitochondria, DNA-Binding Proteins, Plant Leaves, Repressor Proteins, Basic-Leucine Zipper Transcription Factors, Mutation, Mitochondrial carrier family, Carrier Proteins, Succinate transporter
Saccharomyces cerevisiae Proteins, Arabidopsis thaliana, Recombinant Fusion Proteins, Molecular Sequence Data, Arabidopsis, Anaplerotic reaction, Mitochondrial Proteins, Fumarates, Gene Expression Regulation, Plant, Amino Acid Sequence, Cloning, Molecular, Promoter Regions, Genetic, Phylogeny, Arabidopsis Proteins, Genetic Complementation Test, Gluconeogenesis, Mitochondria, DNA-Binding Proteins, Plant Leaves, Repressor Proteins, Basic-Leucine Zipper Transcription Factors, Mutation, Mitochondrial carrier family, Carrier Proteins, Succinate transporter
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).51 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!