Identification and characterization of a Drosophila nuclear receptor with the ability to inhibit the ecdysone response.
Copyright policy )Identification and characterization of a Drosophila nuclear receptor with the ability to inhibit the ecdysone response.
In a search for retinoid X receptor-like molecules in Drosophila, we have identified an additional member of the nuclear receptor superfamily, XR78E/F. In the DNA-binding domain, XR78E/F is closely related to the mammalian receptor TR2, as well as to the nuclear receptors Coup-TF and Seven-up. We demonstrate that XR78E/F binds as a homodimer to direct repeats of the sequence AGGTCA. In transient transfection assays, XR78E/F represses ecdysone signaling in a DNA-binding-dependent fashion. XR78E/F has its highest expression in third-instar larvae and prepupae. These experiments suggest that XR78E/F may play a regulatory role in the transcriptional cascade triggered by the hormone ecdysone in Drosophila.
- University of California, San Diego United States
Ecdysone, Genomic Library, Time Factors, Receptors, Retinoic Acid, Molecular Sequence Data, Genes, Insect, Sequence Analysis, DNA, Blotting, Northern, Recombinant Proteins, Blotting, Southern, Ecdysterone, Retinoid X Receptors, Animals, Drosophila, Tissue Distribution, Amino Acid Sequence, In Situ Hybridization, Protein Binding, Signal Transduction, Transcription Factors
Ecdysone, Genomic Library, Time Factors, Receptors, Retinoic Acid, Molecular Sequence Data, Genes, Insect, Sequence Analysis, DNA, Blotting, Northern, Recombinant Proteins, Blotting, Southern, Ecdysterone, Retinoid X Receptors, Animals, Drosophila, Tissue Distribution, Amino Acid Sequence, In Situ Hybridization, Protein Binding, Signal Transduction, Transcription Factors
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