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Biophysical Journal
Article . 2013
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Biophysical Journal
Article . 2013 . Peer-reviewed
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Functional Role of Two Protein-Associated Ubiquinone Molecules (Q-NF and Q-NS) for the Proton-Pumping Mechanism in Bovine Heart Complex I (NADH-Ubiquinone Oxidoreductase)

Authors: S. Tsuyoshi Ohnishi; Tomoko Ohnishi;

Functional Role of Two Protein-Associated Ubiquinone Molecules (Q-NF and Q-NS) for the Proton-Pumping Mechanism in Bovine Heart Complex I (NADH-Ubiquinone Oxidoreductase)

Abstract

We had characterized the function of two distinct protein-associated ubisemiquinone molecules in the proton-pumping mechanism in complex I (NADH-UQ oxidoreductase).We constructed most of the frame work of our proton-pumping hypothesis, utilizing EPR techniques before the X-ray structures of bacterial and mitochondrial complex I were reported by Sazanov's group and Brandt and his collaborators, respectively. The fast relaxing semiquinone (SQ-Nf) signal is extremely sensitive to the proton motive force (ΔP) imposed to the energy transducing membrane, strongly indicating its direct involvement in proton-pumping mechanism. Slow relaxing semiquinone (SQ-Ns) is not sensitive to ΔP. Although they show identical piericidin A sensitivity, they differ in rotenone sensitivity considerably as well as their SQ binding subunits. These differences were exploited using tightly coupled bovine heart submitochondrial particles with a high respiratory control ratio (>8).We determined the center-to-center distance of 12 A between SQ-Nf and its direct electron donor, iron-sulfur cluster N2 based on their spin-spin interaction analysis. We have extended this work using reconstituted bovine heart complex I proteoliposomes which shows a respiratory control ratio >5. Our recent Q-band (33.9 GHz) EPR analysis of SQ-Nf and SQ-Ns spectra in the reconstituted proteoliposome system also supports our two-semiquinone model. We will compare our EPR-based model with the X-ray structure based proton-pumping models by Sazanov's group and Brandt with his collaborators.

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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