Estimation of the in situ hybridization expression of IL-4and IL-10 in patients with malignant breast cancer.A technique utilizing in situ hybridization (ISH) was performed to detect and determine the in situ expression of IL-4 and IL-10 mRNA using paraffin embedded sections .Samples obtained from archive of department of pathology of two hospitals in Baghdad for 60 patients, who were divided into two groups:30 with malignant breast cancer (group-1) and 30 woman with nonmalignant as a control group (group-2).The levels of the in situ expression of both IL-4 and IL-10 mRNA were found to be a highly significant increased in group 1 as compared with group 2 (p<0.001), with no significant correlation between IL-4 and IL-10 (p>0.05) in all studied group.The increasing expression of IL-4 and IL-10 in malignant breast cancer tissue might be influence in the pathogenesis of malignant breast cancer.