The authors devised a cytotoxic assay based on cytofluorometric analysis of target surface markers in order to compare lysis exerted in vitro by cytotoxic T lymphocytes (CTLs) on different cell subsets in the context of a single lymphoid target cell population. Using this assay, the authors evaluated when oncorna virus‐infected lymphocytes become a suitable target for virus‐specific T cell effectors. A lymphocyte population from Moloney‐murine leukaemia virus (M‐MuLV)‐infected (carrier) mice, in which the proliferation of selective Vβ T‐cell receptor (TCR) families was induced in response to Mlsa encoded antigens, was utilized as a target. The authors observed that a virus‐specific T cell clone exerted lytic activity preferentially against activated cell subsets. Moreover, virus‐specific CTLs generated in mixed leucocyte tumour cell cultures (MLTC) were also able to impair the concomitant anti‐Mlsa response of lymphocytes from M‐MuLV carrier mice. It was found that the proliferative status of oncorna virus‐infected target cells played an important role in limiting the in vitro efficacy of the immune response, and it is speculated that this phenomenon might represent an in vivo escape mechanism from immunosurveillance.